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| Simental-Mendía,M.; Lara-Arias,J.; Álvarez-Lozano,E.; Said-Fernández,S.; Soto-Domínguez,A.; Padilla-Rivas,G. R.; Martínez-Rodríguez,H. G.. |
| Damage to cartilage causes a loss of type II collagen (Col-II) and glycosaminoglycans (GAG). To restore the original cartilage architecture, cell factors that stimulate Col-II and GAG production are needed. Insulin-like growth factor I (IGF-I) and transcription factor SOX9 are essential for the synthesis of cartilage matrix, chondrocyte proliferation, and phenotype maintenance. We evaluated the combined effect of IGF-I and SOX9 transgene expression on Col-II and GAG production by cultured human articular chondrocytes. Transient transfection and cotransfection were performed using two mammalian expression plasmids (pCMV-SPORT6), one for each transgene. At day 9 post-transfection, the chondrocytes that were over-expressing IGF-I/SOX9 showed 2-fold increased... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Type II collagen; Glycosaminoglycans; IGF-I/SOX9 transgenes; Human chondrocytes; Articular cartilage; Cotransfection. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001201063 |
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| Mohammadie,Zari Majidi; Parivar,Kazem; Shahri,Nasser Mahdavi; Fereidoni,Masoud; Hayati-Roodbari,Nasim. |
| ABSTRACT Nanotubes with their unique properties have diversified mechanical and biological applications. Due to similarity of dimensions with extracellular matrix (ECM) elements, these materials are used in designing scaffolds. In this research, Carboxylated Single-Wall Carbon Nanotubes in optimization of decellularized scaffold of bovine articular cartilage was used. At first, the articular cartilage was decellularized. Then the scaffolds were analyzed in: (i) decellularized scaffolds, and (ii) scaffolds plunged into homogenous suspension of nanotubes in distilled water, were smeared with Carboxylated-SWCNT. The tissue rings derived from the rabbit's ear were assembled with reinforced scaffolds and they were placed in a culture media for 15 days. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Articular cartilage; Carboxylated-SWCNT; Extracellular matrix; Natural scaffold; Tissue engineering. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100607 |
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| Fialho,L.; Rafael,A.; Cabrita,A.S.; Pereira,A.M.F.; Capela e Silva,F.. |
| Avaliou-se o efeito do tirame, ditiocarbamato largamente utilizado na agricultura como antifúngico e repelente de roedores, na ossificação endocondral de mamíferos, usando, como modelo, ratos Wistar. Não foram observadas lesões na cartilagem articular, nem nas placas de crescimento, o que pode ser atribuído à dose utilizada e à duração do ensaio. A diminuição da altura da placa de crescimento nos animais aos quais foi administrado o tirame parece traduzir o atraso verificado no crescimento em geral, e não um efeito específico na cartilagem, uma vez que as diferentes zonas da placa epifisária mantiveram as proporções dos animais do grupo-controle. Embora não tenham sido verificados, no presente trabalho, os efeitos registrados para outras espécies nos... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rat; Dithiocarbamate; Thiram; Growth plate; Articular cartilage. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000100036 |
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| Kunz,R.I.; Coradini,J.G.; Silva,L.I.; Bertolini,G.R.F.; Brancalhão,R.M.C.; Ribeiro,L.F.C.. |
| A sprained ankle is a common musculoskeletal sports injury and it is often treated by immobilization of the joint. Despite the beneficial effects of this therapeutic measure, the high prevalence of residual symptoms affects the quality of life, and remobilization of the joint can reverse this situation. The aim of this study was to analyze the effects of immobilization and remobilization on the ankle joint of Wistar rats. Eighteen male rats had their right hindlimb immobilized for 15 days, and were divided into the following groups: G1, immobilized; G2, remobilized freely for 14 days; and G3, remobilized by swimming and jumping in water for 14 days, performed on alternate days, with progression of time and a series of exercises. The contralateral limb was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Synovial joint; Remobilization in aquatic environment; Morphology; Articular cartilage; Synovial membrane. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001000842 |
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| Filgueiras,R.R.; Del Carlo,R.J.; Alves,N.P.; Viloria,M.I.V.; McManus,C.M.; Castro,M.B.; Filgueiras,F.P.F.; Monteiro,B.S.; Almeida,R.M.; Andrade,R.R.. |
| Chondrocytes obtained from stifle joint of New Zealand White rabbits were cultivated. Half of cells were maintained in culture for later implantation and the others frozen during six months to evaluate viability. A circular osteochondral defect was created in the right stifle of other twenty seven rabbits. The control group (CG) received no treatment. The thawed (TH) and fresh (FH) heterologous groups received, respectively, an implant of cultivated thawed or fresh heterologous chondrocytes associated with platelet rich plasma (PRP). The CG group showed greatest pain and lameness compared to the other groups seven days after the implantation. Microscopically, at 45 and 90 days, the TH and FH groups showed filling with cartilaginous tissue containing... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rabbits; Articular cartilage; Chondrocyte implantation; Platelet rich plasma; Type II collagen. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000100024 |
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| Ma,C.H.; Lv,Q.; Yu,Y.X.; Zhang,Y.; Kong,D.; Niu,K.R.; Yi,C.Q.. |
| We aimed to investigate the effects of an anti-tumor necrosis factor-α antibody (ATNF) on cartilage and subchondral bone in a rat model of osteoarthritis. Twenty-four rats were randomly divided into three groups: sham-operated group (n=8); anterior cruciate ligament transection (ACLT)+normal saline (NS) group (n=8); and ACLT+ATNF group (n=8). The rats in the ACLT+ATNF group received subcutaneous injections of ATNF (20 μg/kg) for 12 weeks, while those in the ACLT+NS group received NS at the same dose for 12 weeks. All rats were euthanized at 12 weeks after surgery and specimens from the affected knees were harvested. Hematoxylin and eosin staining, Masson's trichrome staining, and Mankin score assessment were carried out to evaluate the cartilage status and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Anti-tumor necrosis factor-α antibody (ATNF); Osteoarthrosis; Matrix metalloproteinase (MMP)-13; Articular cartilage; Subchondral bone. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001000001 |
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